Establishment and characterization of a rat pancreatic stellate cell line by spontaneous immortalization
نویسندگان
چکیده
منابع مشابه
Generation and characterization of immortalized rat pancreatic stellate cells.
Pancreatic stellate cells (PSCs) are involved in, among other things, the pathogenesis of pancreatic fibrosis. Here, we present the generation of immortalized PSCs 7 and 14 days after isolation by retroviral gene transfer of the SV40 large T antigen encoding region. Propagated cell lines [large T immortalized cells (LTC)-7, LTC-14] retained characteristics of primary cells in terms of morpholog...
متن کاملGEM Cell Line Establishment, Characterization, and Analysis
Spring 2013 | Volume 7 Columbia Undergraduate Science Journal 4 GEM Cell Line Establishment, Characterization, and Analysis Daniel Chester Abate-Shen Lab, Columbia University, New York NY 10027 Abstract The use of in-vitro cell lines in conjunction with in-vivo mouse models is a common practice in the study of cancer. Culturing cells with the same genotypes as the specific strains of mice being...
متن کاملEstablishment and characterization of a differentiating myeloid cell line obtained from a rat myelomonocytic leukemia.
A long-term suspension culture line (c-WRT-7) was successfully established from a transplantable myelomonocytic leukemia induced by a neonatal injection of Rauscher leukemia virus in a WKA/Hok rat. A c-WRT-7 cell line was capable of being transplanted into syngeneic rats, and when transplanted, increased numbers of macrophage-like cells were observed in the peripheral blood of rats after i.v. i...
متن کاملEstablishment and characterization of a virus-free chick cell line.
A line of chick embryo cells (CEC) was obtained from CEC treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The cells, designated CHCC-OU2, were contact-inhibited, formed no colony in soft agar and did not produce tumors when inoculated into syngeneic chickens. The electron microscopic examination and reverse transcriptase assay showed no virus production from the cells. Subgroup A avian...
متن کاملEstablishment and characterization of a human cholangiocarcinoma cell line.
Cholangiocarcinoma (CC) is a rare malignant tumor arising from the biliary tract. The disease is notoriously difficult to diagnose and is usually fatal due to its late clinical presentation and the lack of effective non-surgical therapeutic strategies. To date, little is known about the cancer biology of the disease and the establishment and characterization of only a few CC cell lines have bee...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: World Journal of Gastroenterology
سال: 2003
ISSN: 1007-9327
DOI: 10.3748/wjg.v9.i12.2751